In vitro digestion of acetylated casein by pepsin-pancreatin.
نویسندگان
چکیده
منابع مشابه
Casein phosphopeptides from casein micelles by successive digestion with pepsin and trypsin.
When milk is ingested, casein micelles will be successively digested by pepsin in the stomach and trypsin in the intestine. Therefore, we digested casein micelles successively with pepsin at pH 4.0 and trypsin at pH 7.0, and recovered casein phosphopeptides (CPP) as CPP-calcium phosphate (CP) complexes. The CPP-CP complexes contained 248 mg of calcium/g peptides and 175 mg of inorganic phosphor...
متن کاملStructure of an Exophthalmos-producing Thyrotropin by Partial Pepsin Digestion
Previously reported experiments (Winand, R. J., and Kohn, L. D. (1970) J. Biol. Chem. 245, 967-975; Kohn, L. D., and Winand, R. J. (1971) J. Biol. Chem. 246, 6570-6575) have demonstrated that partial pepsin digestion of bovine thyrotropin preparation yields a fragment of the thyrotropin molecule which is exophthalmogenic but has negligible or no thyroid-stimulating activity. In the present repo...
متن کاملLimited pepsin digestion of human plasma albumin.
Limited pepsin digestion of human plasma albumin at pH 3.5 and 0 degrees in the presence of octanoate caused cleavage at residue 307 of the albumin molecule to yield two fragments. Thw two fragments corresponding to the NH2- and the COOH-terminal halves of the molecule were isolated in yields of about 15%. The COOH-terminal fragment is a mixture in which about 85% of the molecules had an additi...
متن کاملPepsin Digestion of Rabbit and Sheep Antibodies
7S sheep antibody is similar to 7S rabbit antibody, insofar as it too can be digested with pepsin to yield a 5S fragment still capable of precipitating homologous antigen. The 5S fragment from rabbit as well as sheep antibody is capable of fixing guinea pig complement. However, this fixation differs from the fixation by 7S antibody, since preformed antibody-antigen aggregates containing either ...
متن کاملThe influence of selected cardiovascular and antidiabetic drugs on pepsin activity in vitro digestion.
The aim of this study was to assess the influence of selected cardiovascular and antidiabetic drugs on in vitro pepsin activity. A total of 13 drugs were analyzed at one concentration (one tablet of drug in 50 mL of solution with deionized water). The enzyme activity was determined by the Folin method based on the reaction of tyrosine with the Folin reagent. It was found that ACE inhibitors (ap...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1978
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.42.2173